Hypothesis: If flies with different genes are mated, the dominant genes will be more prominant within the population. Materials:
Procedure: Obtain a vial of parental fl ies from your teacher. Anesthetize the flies asinstructed by your teacher and practice looking at the fl ies under the dissecting microscope. You should note the sex differences. Also note the type of mutation written on the vial. 1. Obtain a vial of F1 fl ies from your teacher.
2. Place 5-6 male and female F1 fl ies into a fresh vial with media. 3. After 10 days, remove the F1 parents and discard into the fl y morgue. 4. The F2 generation fl ies will begin to emerge within several days.
section titled Using EDVOTEK® BugOutTM solution.
5. Analyze the data using the chi-squared statistic as previously described. Data: (all chi-squares are based off of data recieved from the virtual lab). STUDY QUESTIONS:
11. -- Answers for 11 are located in Data section above --
CONCLUSION: My hypothesis "If flies with different genes are mated, the dominant genes will be more prominant within the population." was proved correct in my data of the different crosses. In all of the crosses the dominant alleles have a higher frequency than the recessive. I knew this because the flies showed a majority of dominant phenotypes over the recessive phenotypes. A source of error was not mating the flies correctly and leaving them in the freezer for too long. This lab taught me a lot about genes and punnett squares, as well as how to apply that to chi squared and creating null hypotheses.
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